TīmeklisHuman microsome pools are fully characterized (K m and V max) according to GLP standards for major cytochrome P450 activities and select Phase II enzymes using FDA recommended substrates (Tables 2-3). Table 1. Relevant applications for liver subcellular fractions. Table 2. Cytochrome P450 and Phase II enzymes tested in … TīmeklisLiver S9 fractions prepared from a pool of male Sprague Dawley rats. Total protein concentration: 20 mg/ml We provide liver subcellular fractions from a variety of tox …
1 Draft OECD Guidance document
TīmeklisBecause RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies. TīmeklisS9 is a liver extract (e.g. rat, hamster) that contains active liver enzymes (P450 activity) simulating the hepatic metabolism in in vitro assays. The P450 activity in S9 can be … southlands hotel scarborough reviews
The utility of metabolic activation mixtures containing human
TīmeklisPooled human liver S9 fractions. Total protein concentration: 20 mg/ml. We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Tīmeklis115 14. The major advantage using liver S9 sub-cellular fractions compared to hepatocytes is the 116 ease of preparation and simpler shipment and storage conditions (i.e., shipment on dry ice and 117 storage at -80°C for S9 sub-cellular fractions vs. shipment in liquid nitrogen and storage in liquid Tīmeklis1999. gada 2. janv. · The mutagenicity of 13 chemicals was compared using human liver S9 or liver S9 prepared from male Sprague–Dawley rats either non-treated (R-n) or … teaching jobs in hull