Myc shrna
WebshRNA プラスミドは、 ノックアウト最大化のために設計される3から5種類のターゲット特異的な19-25ntのレンチウイルスベクタープラスミドのプールから構成されます。 shRNA配列 N-Myc siRNA Gene Silencer sequences Web17 aug. 2024 · FUS targeting shRNA was TRCN0000039824. c-Myc targeting shRNA was TRCN0000039642. 5′-AGCGUGUAGCUAGCAGAGG-3′ was used as negative control sequence. Retroviral construct and transfection. The ...
Myc shrna
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WebFigure 1 Knockdown of PCGF1 by lentivirus-mediated short hairpin RNA (shRNA)-induced cell cycle arrested at G0/G1 phase and attenuation of proliferation in U87 cells.Notes: (A) Expression analysis of PCGF1 mRNA by RT-qPCR analysis.(B) Expression of PCGF1 proteins in infected cells was analyzed by Western blot.(C) Cell cycle distribution of U87 … Web1 jan. 2015 · MYC is aberrantly expressed in a variety of different forms of cancer and plays an important role in the biology of various lymphoma subtypes such as Burkitt lymphoma …
Web21 mrt. 2024 · Background Isocitrate dehydrogenase-2 (IDH2) is a mitochondrial enzyme that catalyzes the metabolic conversion between isocitrate and alpha-ketoglutarate (α-KG) in the TCA cycle. IDH2 mutation is an oncogenic event in acute myeloid leukemia (AML) due to the generation of 2-hydroxyglutarate. However, the role of wild-type IDH2 in AML … Web10 aug. 2024 · shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to …
MYC is a key oncogene that controls several cellular pathways relevant to cancer, including cell cycle entry, ribosome synthesis, and various metabolic processes. Indeed, overexpression of MYC is one of the most common drivers of many cancer types30, including AML31. As shown above, KD of RBM25 … Meer weergeven In order to identify splicing factors that functionally affect AML biology, we conducted an in vivo pooled shRNA screen in the … Meer weergeven Having shown that RBM25 acts as a tumor suppressor in murine AML, we now wanted to test if we could expand this to a human setting. To do so, we first transduced … Meer weergeven Among our validated tumor suppressor hits, Rbm25 KD yielded the most prominent effect on Lp30 AML cells compared to c-Kit+ control cells (Fig. 1d, e, see … Meer weergeven In order to gain mechanistic insights into RBM25 function, we next performed RNA-sequencing (RNA-seq) on U937 cells transduced with scrambled or RBM25-specific shRNAs and obtained a prominent separation … Meer weergeven WebUpon transient transfection with plasmid-encoding shRNA, it was found that expression of c-Myc decreased in shRNA-transfected cells, and the downregulation of c-Myc inhibited …
Web13 apr. 2024 · Silencing TMEM40 with shRNA inhibited the invasion, migration and proliferation, promoted apoptosis and led to a G0/G1 cell cycle arrest in CC cell lines. Silence of TMEM40 downregulated the expression of c-MYC, Cyclin D1, matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9), but in contrast, …
WebMyc shRNA Alt name Myc Species H. sapiens (human) Insert Size (bp) 64 Entrez Gene MYC ( a.k.a. MRTL, MYCC, bHLHe39, c-Myc) Cloning Information Cloning method Restriction Enzyme 5′ cloning site BglII? (not destroyed) 3′ cloning site HindIII (not destroyed) 5′ sequencing primer H1 (Common Sequencing Primers) Resource Information body shops berlin mdWeb1 jul. 2024 · EMS connects c-Myc to cell cycle control and tumorigenesis. ( A) A549 cells were infected with lentiviruses expressing control shRNA (shctrl), c-Myc shRNA (shc-Myc), or both c-Myc shRNA and EMS. Forty-eight hours later, cells were incubated with actinomycin D (2 μg/mL) for the indicated periods of time. body shops billings montanaWeb20 sep. 2013 · YAP or c-Myc-specific shRNAs with high knockdown efficiency ( Fig. 1A and B) were used to silence protein expression in both Bel-7402 and SMMC-7721 cells. We found that inhibition of either YAP or c-Myc decreased cell proliferation compared to control, as measured by a MTT-based assay (Fig. 1 C). glen willow apartments seat pleasant mdWebSince the differential protein expression of MYC is a regulator of the RU/RR dichotomy and is higher in RR cells, we asked if MYC regulates the autophagy-mediated cytoprotective effect. Inhibition of MYC in RR cells using shRNA significantly blunted crizotinib-induced autophagic response and effectively suppressed this cytoprotective effect. glen willow apartments corpus christi texasWeb4 feb. 2024 · Two shRNA against ACTL6A and one shRNA against MYC in pSuper-retro-neo vector were purchased from Sigma-Aldrich. Transfection of plasmids was performed using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, California, USA) according to the manufacturer’s instructions. body shops billingsWeb12 jun. 2024 · The sEVs of Myc-manipulated SCLC cells were isolated using total exosome isolation reagents (from cells) (Life Technologies, USA). The sEVs were harvested from … glen willow apartments houstonWeb1 jul. 2011 · In this study, we used a lentivirus vector-based RNAi approach which expresses short hairpin RNA (shRNA) to knockdown MYCN in neuroblastoma cell lines IMR-32 and … glen willow apartments san jose